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The resultant polyprenol(s) is a substrate for steroid 5-α reductase 3 (also called polyprenol reductase) which is encoded by the SRD5A3 gene. Steroid 5-α reductase 3 belongs to the polyprenol reductase sufamily of the steroid 5-α reductase family. The SRD5A3 encoded enzyme reduces the carbon-carbon double bond closest to the hydroxyl end of the polyprenol generating dolichol. In addition to participating in the synthesis of dolichol the SRD5A3 encoded enzyme synthesizes 5-α-dihydrotestosterone from testosterone. The SRD5A3 gene is located on chromosome 4q12 and is composed of 6 exons that encode a 318 amino acid protein. Mutations in the SRD5A3 gene are associated with the congenital disorder of glycosylation (CDG) identified as CDG-1q (SRD5A3-CDG).

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